IRCMS International Research Center for Medical Sciences

Research

Kenichi Miharada
Ph.D.
Professor

ACHIEVEMENT

Selected Publications

  1. Soboleva S, Miharada K*.
    Induction of enucleation in primary and immortalized erythroid cells.
    Int J Hematol 2022 May 24. Online ahead of print. Review.
  2. Soboleva S, Åkerstrand H, Miharada K*.
    Transcriptomic analysis of functional diversity of human umbilical cord blood hematopoietic stem/progenitor cells in erythroid differentiation.
    Int J Hematol 2022 Apr;115(4):481-488.
  3. Soboleva S, Kurita R, Kajitani N, Åkerstrand H, Miharada K*.
    Establishment of an immortalized human erythroid cell line sustaining differentiation potential without inducible gene expression system.
    Hum Cell 2022 Jan;35(1):408-417.
  4. Koide S, Sigurdsson V, Radulovic V, Saito K, Zheng Z, Lang S, Soneji S, Iwama A, Miharada K*.
    CD244 expression represents functional decline of murine hematopoietic stem cells after in vitro culture.
    iScience 2021 Dec 10;25(1):103603. eCollection 2022 Jan 21.
  5. Soboleva S, Kurita R, Ek F, Åkerstrand H, Silvério-Alves R, Olsson R, Nakamura Y, Miharada K*.
    Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines.
    Commun Biol. 2021 Jun 3;4:677.
  6. Mansell E, Sigurdsson V, Deltcheva E, Brown J, James C, Miharada K, Soneji S, Larsson J, Enver T*.
    Mitochondrial potentiation ameliorates age related heterogeneity in hematopoietic stem cell function.
    Cell Stem Cell 2021 Feb 4;28(2):241-256.
  7. Sigurdsson V, Haga Y, Takei H, Mansell E, Okamatsu-Haga C, Suzuki M, Radulovic V, van der Garde M, Koide S, Soboleva S, Gåfvels M, Nittono H, Ohara A, Miharada K*.
    Induction of blood-circulating bile acids supports recovery from myelosuppressive chemotherapy.
    Blood Adv. 2020 May 12;4(9):1833-1843.
  8. Radulovic V, van der Garde M, Koide S, Sigurdsson V, Lang S, Kaneko S, Miharada K*.
    Junctional Adhesion Molecule 2 Represents a Subset of Hematopoietic Stem Cells with Enhanced Potential for T Lymphopoiesis.
    Cell Rep. 2019 Jun 4;27(10):2826-2836.
  9. Sigurdsson V, Miharada K*.
    Regulation of unfolded protein response in hematopoietic stem cells.
    Int J Hematol. 2018 Jun;107(6):627-633. Review.
  10. Komorowska K, Doyle A, Wahlestedt M, Subramaniam A, Debnath S, Chen J, Soneji S, Van Handel B, Mikkola H, Miharada K, Bryder D, Larsson L, Magnusson M*.
    Hepatic Leukemia Factor maintains quiescence of hematopoietic stem cells and protects the stem cell pool during regeneration.
    Cell Rep. 2017 Dec 19;21(12):3514-3523.
  11. Rörby E, Billing M, Dahl M, Warsi S, Andradottir S, Miharada K, Siva K, Jönsson JI, Blank U, Karlsson G, Karlsson S*.
    The stem cell regulator PEDF is dispensable for maintenance and function of hematopoietic stem cells.
    Sci Rep. 2017 Aug 31;7(1):10134.
  12. Prost G, Braun S, Hertwig F, Winkler M, Jagemann L, Nolbrant S, Leefa IV, Offen N, Miharada K, Lang S, Artner I, Nuber UA*.
    The putative tumor suppressor gene EphA7 is a novel BMI-1 target.
    Oncotarget 2016 Sep 6;7(36):58203-58217.
  13. Sigurdsson V, Takei H, Soboleva S, Radulovic V, Galeev R, Siva K, Leeb-Lundberg LMF, Iida T, Nittono H, Miharada K*.
    Bile acids protect expanding hematopoietic stem cells from unfolded protein stress in fetal liver.
    Cell Stem Cell 2016 Apr 7;18(4):522-32.
  14. Miharada K*, Sigurdsson V, Karlsson S*. (*Co-corresponding author)
    Dppa5 improves hematopoietic stem cell activity by reducing endoplasmic reticulum stress.
    Cell Rep. 2014 June 12;7(5):1381-92.
  15. Ozen I, Deierborg T, Miharada K, Padel T, Englund E, Genové G, Paul G*.
    Brain pericytes acquire a microglial phenotype after stroke.
    Acta Neuropathol. 2014 Sep;128(3):381-96.   
  16. Karlsson G, Rörby E, Pina C, Soneji S, Reckzeh K, Miharada K, Karlsson C, Guo Y, Fugazza C, Gupta R, Martens JH, Stunnenberg HG, Karlsson S, Enver T*.
    The Tetraspanin CD9 affords high-purity capture of all murine hematopoietic stem cells.
    Cell Rep. 2013 Aug 29;4(4):642-8.
  17. Kurita R, Suda N, Sudo K, Miharada K, Hiroyama T, Miyoshi H, Tani K, Nakamura Y*.
    Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells.
    PLoS One 2013;8(3):e59890.
  18. Miharada K*, Karlsson G, Rehn M, Rörby E, Siva K, Cammenga J, Karlsson S*. (*Co-corresponding author)
    Hematopoietic stem cells are regulated by Cripto, as an intermediary of HIF-1α in the hypoxic bone marrow niche.
    Ann N Y Acad Sci. 2012; 1266:55-62. Review. 
  19. Miharada K, Nakamura Y*.
    In vitro production of enucleated red blood cells from hematopoietic stem and progenitor cells.
    Methods Mol Biol. 2012; 879:505-12. Review.
  20. Siva K, Jaako P, Miharada K, Rörby E, Ehinger M, Karlsson G, Karlsson S*.
    SPARC is dispensable for murine hematopoiesis, despite its suspected pathophysiological role in 5q-myelodysplastic syndrome.
    Leukemia 2012 Nov;26(11):2416-9.
  21. Miharada K*, Karlsson G, Rehn M, Rörby E, Siva K, Cammenga J, Karlsson S*. (*Co-corresponding author)
    Cripto regulates hematopoietic stem cells as a hypoxic-niche-related factor through cell surface receptor GRP78.
    Cell Stem Cell 2011 Oct 4;9(4):330-44.
  22. Hiroyama T, Miharada K, Kurita R, Nakamura Y*.
    Plasticity of cells and ex vivo production of red blood cells.
    Stem Cells Int. 2011;2011:195780.
  23. Singbrant S, Karlsson G, Ehinger M, Olsson K, Jaako P, Miharada K, Stadtfeld M, Graf T, Karlsson S*.
    Canonical BMP signaling is dispensable for hematopoietic stem cell function in both adult and fetal liver hematopoiesis, but essential to preserve colon architecture.
    Blood 2010 Jun 10;115(23):4689-98.
  24. Ishigaki T, Sudo K, Hiroyama T, Miharada K, Ninomiya H, Chiba S, Nagasawa T, Nakamura Y*.
    Human hematopoietic stem cells can survive in vitro for several months.
    Adv Hematol. 2009;2009:936761.
  25. Hiroyama T, Miharada K, Sudo K, Danjo I, Aoki N, Nakamura Y*.
    Establishment of mouse embryonic stem cell-derived erythroid progenitor cell lines able to produce functional red blood cells.
    PLoS One 2008 Feb 6;3(2):e1544.
  26. Miharada K, Hiroyama T, Sudo K, Danjo I, Nagasawa T, Nakamura Y*.
    Lipocalin 2-mediated growth suppression is evident in human erythroid and monocyte/macrophage lineage cells.
    J Cell Physiol. 2008 May;215(2):526-37.
  27. Hiroyama T, Sudo K, Aoki N, Miharada K, Danjo I, Fujioka T, Nagasawa T, Nakamura Y*.
    Human umbilical cord-derived cells can often serve as feeder cells to maintain primate embryonic stem cells in a state capable of producing hematopoietic cells.
    Cell Biol Int. 2008 Jan;32(1):1-7.
  28. Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y*.
    Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.
    Stem Cells 2007 Jul;25(7):1610-7.
  29. Miharada K, Hiroyama T, Sudo K, Nagasawa T, Nakamura Y*.
    Efficient enucleation of erythroblasts differentiated in vitro from hematopoietic stem and progenitor cells.
    Nat Biotechnol. 2006 Oct;24(10):1255-6.
  30. Hiroyama T, Miharada K, Aoki N, Fujioka T, Sudo K, Danjo I, Nagasawa T, Nakamura Y*.
    Long-lasting in vitro hematopoiesis derived from primate embryonic stem cells.
    Exp Hematol. 2006 Jun;34(6):760-9.
  31. Miharada K, Hiroyama T, Sudo K, Nagasawa T, Nakamura Y*.
    Refinement of cytokine use in the in vitro expansion of erythroid cells.
    Hum Cell 2006 Feb;19(1):30-7.
  32. Miharada K, Hiroyama T, Sudo K, Nagasawa T, Nakamura Y*.
    Lipocalin 2 functions as a negative regulator of red blood cell production in an autocrine fashion.
    FASEB J. 2005 Nov;19(13):1881-3.