PIs
Kenichi Miharada
Ph.D.
Professor
ACHIEVEMENT
Selected Publications
- Saito K#, van der Garde M#, Umemoto T, Miharada N, Sjöberg J, Sigurdsson V, Shirozu H, Kamei S, Radulovic V, Suzuki M, Nakano S, Lang S, Hansson J, Olsson ML, Minami T, Gouras G, Flygare J, Miharada K*.
Lipoprotein metabolism mediates hematopoietic stem cell responses under acute anemic conditions.
Nat Commun. 2024 15:8131. - Suzuki M, Nakano S, Miharada N, Takei H, Prabhala P, van der Garde M, Müller C, Sigurdsson V, Aerken M, Saito K, Koide S, Westergren-Thorsson G, Magnusson M, Kakiyama G, Nittono H, Miharada K*.
Maternal sterol 27-hydroxylase is crucial for securing fetal development.
bioRxiv. 2023.11.08.566330. - Ma W, Arima Y, Umemoto T, Yokomizo T, Xu Y, Miharada K, Tanaka Y, Suda T*.
Metabolic regulation in erythroid differentiation by systemic ketogenesis in fasted mice.
Exp Hematol. 2023 Oct 26:S0301-472X(23)01739-3. - Rydström A, Grahn THM, Niroula A, Mansell E, van der Garde M, Pertesi M, Subramaniam A, Soneji S, Zubarev R, Enver T, Nilsson B, Miharada K*, Larsson J*, Karlsson S*. (*Co-corresponding authors).
Functional and molecular profiling of hematopoietic stem cells during regeneration.
Exp Hematol. 2023 Nov;127:40-51. - Zimmermannova O, Ferreira AG, Ascic E, Velasco Santiago M, Kurochkin I, Hansen M, Met Ö, Caiado I, Shapiro IE, Michaux J, Humbert M, Soto-Cabrera D, Benonisson H, Silvério-Alves R, Gomez-Jimenez D, Bernardo C, Bauden M, Andersson R, Höglund M, Miharada K, Nakamura Y, Hugues S, Greiff L, Lindstedt M, Rosa FF, Pires CF, Bassani-Sternberg M, Svane IM, Pereira CF*.
Restoring tumor immunogenicity with dendritic cell reprogramming.
Sci Immunol. 2023 Jul 14;8(85):eadd4817. - Rydström A, Mansell E, Sigurdsson V, Sjöberg J, Soneji S, Miharada K, Larsson J*.
MAC-1 marks a quiescent and functionally superior HSC subset during regeneration.
Stem Cell Reports. 2023 Mar 14;18(3):736-748. - Yang C, Yokomori R, Chua LH, Tan SH, Tan DQ, Miharada K, Sanda T, Suda T*.
Mitochondria transfer mediates stress erythropoiesis by altering the bioenergetic profiles of early erythroblasts through CD47.
J Exp Med. 2022 Dec 5;219(12):e20220685. - Soboleva S, Miharada K*.
Induction of enucleation in primary and immortalized erythroid cells.
Int J Hematol 2022 Aug;116(2):192-198. Review. - Soboleva S, Åkerstrand H, Miharada K*.
Transcriptomic analysis of functional diversity of human umbilical cord blood hematopoietic stem/progenitor cells in erythroid differentiation.
Int J Hematol 2022 Apr;115(4):481-488. - Soboleva S, Kurita R, Kajitani N, Åkerstrand H, Miharada K*.
Establishment of an immortalized human erythroid cell line sustaining differentiation potential without inducible gene expression system.
Hum Cell 2022 Jan;35(1):408-417. - Koide S#, Sigurdsson V#, Radulovic V, Saito K, Zheng Z, Lang S, Soneji S, Iwama A, Miharada K*.
CD244 expression represents functional decline of murine hematopoietic stem cells after in vitro culture.
iScience 2021 Dec 10;25(1):103603. eCollection 2022 Jan 21. - Soboleva S, Kurita R, Ek F, Åkerstrand H, Silvério-Alves R, Olsson R, Nakamura Y, Miharada K*.
Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines.
Commun Biol. 2021 Jun 3;4:677. - Mansell E, Sigurdsson V, Deltcheva E, Brown J, James C, Miharada K, Soneji S, Larsson J, Enver T*.
Mitochondrial potentiation ameliorates age related heterogeneity in hematopoietic stem cell function.
Cell Stem Cell 2021 Feb 4;28(2):241-256. - Sigurdsson V, Haga Y, Takei H, Mansell E, Okamatsu-Haga C, Suzuki M, Radulovic V, van der Garde M, Koide S, Soboleva S, Gåfvels M, Nittono H, Ohara A, Miharada K*.
Induction of blood-circulating bile acids supports recovery from myelosuppressive chemotherapy.
Blood Adv. 2020 May 12;4(9):1833-1843. - Radulovic V, van der Garde M, Koide S, Sigurdsson V, Lang S, Kaneko S, Miharada K*.
Junctional Adhesion Molecule 2 Represents a Subset of Hematopoietic Stem Cells with Enhanced Potential for T Lymphopoiesis.
Cell Rep. 2019 Jun 4;27(10):2826-2836. - Sigurdsson V, Miharada K*.
Regulation of unfolded protein response in hematopoietic stem cells.
Int J Hematol. 2018 Jun;107(6):627-633. Review. - Komorowska K, Doyle A, Wahlestedt M, Subramaniam A, Debnath S, Chen J, Soneji S, Van Handel B, Mikkola H, Miharada K, Bryder D, Larsson L, Magnusson M*.
Hepatic Leukemia Factor maintains quiescence of hematopoietic stem cells and protects the stem cell pool during regeneration.
Cell Rep. 2017 Dec 19;21(12):3514-3523. - Rörby E, Billing M, Dahl M, Warsi S, Andradottir S, Miharada K, Siva K, Jönsson JI, Blank U, Karlsson G, Karlsson S*.
The stem cell regulator PEDF is dispensable for maintenance and function of hematopoietic stem cells.
Sci Rep. 2017 Aug 31;7(1):10134. - Prost G, Braun S, Hertwig F, Winkler M, Jagemann L, Nolbrant S, Leefa IV, Offen N, Miharada K, Lang S, Artner I, Nuber UA*.
The putative tumor suppressor gene EphA7 is a novel BMI-1 target.
Oncotarget 2016 Sep 6;7(36):58203-58217. - Sigurdsson V, Takei H, Soboleva S, Radulovic V, Galeev R, Siva K, Leeb-Lundberg LMF, Iida T, Nittono H, Miharada K*.
Bile acids protect expanding hematopoietic stem cells from unfolded protein stress in fetal liver.
Cell Stem Cell 2016 Apr 7;18(4):522-32. - Miharada K*, Sigurdsson V, Karlsson S*. (*Co-corresponding author)
Dppa5 improves hematopoietic stem cell activity by reducing endoplasmic reticulum stress.
Cell Rep. 2014 June 12;7(5):1381-92. - Ozen I, Deierborg T, Miharada K, Padel T, Englund E, Genové G, Paul G*.
Brain pericytes acquire a microglial phenotype after stroke.
Acta Neuropathol. 2014 Sep;128(3):381-96. - Karlsson G, Rörby E, Pina C, Soneji S, Reckzeh K, Miharada K, Karlsson C, Guo Y, Fugazza C, Gupta R, Martens JH, Stunnenberg HG, Karlsson S, Enver T*.
The Tetraspanin CD9 affords high-purity capture of all murine hematopoietic stem cells.
Cell Rep. 2013 Aug 29;4(4):642-8. - Kurita R, Suda N, Sudo K, Miharada K, Hiroyama T, Miyoshi H, Tani K, Nakamura Y*.
Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells.
PLoS One 2013;8(3):e59890. - Miharada K*, Karlsson G, Rehn M, Rörby E, Siva K, Cammenga J, Karlsson S*. (*Co-corresponding author)
Hematopoietic stem cells are regulated by Cripto, as an intermediary of HIF-1α in the hypoxic bone marrow niche.
Ann N Y Acad Sci. 2012; 1266:55-62. Review. - Miharada K, Nakamura Y*.
In vitro production of enucleated red blood cells from hematopoietic stem and progenitor cells.
Methods Mol Biol. 2012; 879:505-12. Review. - Siva K, Jaako P, Miharada K, Rörby E, Ehinger M, Karlsson G, Karlsson S*.
SPARC is dispensable for murine hematopoiesis, despite its suspected pathophysiological role in 5q-myelodysplastic syndrome.
Leukemia 2012 Nov;26(11):2416-9. - Miharada K*, Karlsson G, Rehn M, Rörby E, Siva K, Cammenga J, Karlsson S*. (*Co-corresponding author)
Cripto regulates hematopoietic stem cells as a hypoxic-niche-related factor through cell surface receptor GRP78.
Cell Stem Cell 2011 Oct 4;9(4):330-44. - Hiroyama T, Miharada K, Kurita R, Nakamura Y*.
Plasticity of cells and ex vivo production of red blood cells.
Stem Cells Int. 2011;2011:195780. - Singbrant S, Karlsson G, Ehinger M, Olsson K, Jaako P, Miharada K, Stadtfeld M, Graf T, Karlsson S*.
Canonical BMP signaling is dispensable for hematopoietic stem cell function in both adult and fetal liver hematopoiesis, but essential to preserve colon architecture.
Blood 2010 Jun 10;115(23):4689-98. - Ishigaki T, Sudo K, Hiroyama T, Miharada K, Ninomiya H, Chiba S, Nagasawa T, Nakamura Y*.
Human hematopoietic stem cells can survive in vitro for several months.
Adv Hematol. 2009;2009:936761. - Hiroyama T, Miharada K, Sudo K, Danjo I, Aoki N, Nakamura Y*.
Establishment of mouse embryonic stem cell-derived erythroid progenitor cell lines able to produce functional red blood cells.
PLoS One 2008 Feb 6;3(2):e1544. - Miharada K, Hiroyama T, Sudo K, Danjo I, Nagasawa T, Nakamura Y*.
Lipocalin 2-mediated growth suppression is evident in human erythroid and monocyte/macrophage lineage cells.
J Cell Physiol. 2008 May;215(2):526-37. - Hiroyama T, Sudo K, Aoki N, Miharada K, Danjo I, Fujioka T, Nagasawa T, Nakamura Y*.
Human umbilical cord-derived cells can often serve as feeder cells to maintain primate embryonic stem cells in a state capable of producing hematopoietic cells.
Cell Biol Int. 2008 Jan;32(1):1-7. - Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y*.
Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.
Stem Cells 2007 Jul;25(7):1610-7. - Miharada K, Hiroyama T, Sudo K, Nagasawa T, Nakamura Y*.
Efficient enucleation of erythroblasts differentiated in vitro from hematopoietic stem and progenitor cells.
Nat Biotechnol. 2006 Oct;24(10):1255-6. - Hiroyama T, Miharada K, Aoki N, Fujioka T, Sudo K, Danjo I, Nagasawa T, Nakamura Y*.
Long-lasting in vitro hematopoiesis derived from primate embryonic stem cells.
Exp Hematol. 2006 Jun;34(6):760-9. - Miharada K, Hiroyama T, Sudo K, Nagasawa T, Nakamura Y*.
Refinement of cytokine use in the in vitro expansion of erythroid cells.
Hum Cell 2006 Feb;19(1):30-7. - Miharada K, Hiroyama T, Sudo K, Nagasawa T, Nakamura Y*.
Lipocalin 2 functions as a negative regulator of red blood cell production in an autocrine fashion.
FASEB J. 2005 Nov;19(13):1881-3.