Single cell transcriptome

To perform single-cell RNA sequencing, revrese transcription and DNA amplification are necessary. We provide Single Cell Reverse Transcription with Random Displacement Amplification (scRT- RamDA) method. This method enables a 10-100 fold increase in cDNA yield, the highest reproducibility, accuracy, and full-length coverage across transcripts. By using this new technology, you can cyclopedically analyze the transcriptional product in one cell.

For inquiries on collaborative research contact Akifumi Kiyota a-kiyota [at] kumamoto-u.ac.jp

https://icals.kumamoto-u.ac.jp/research-support.php

Single cell proteomics

We provide services of single cell proteomics using the Helios, a CyTOF System for mass spectrometry. The Helios system has 135 detection channels and is able to measure more than 40 markers per cell. Cellular targets are labeled with metal-tagged antibodies. Then they are detected and quantified by time- of -flight mass spectrometry. The high qurity and choice of metal isotopes provide minimal signal overlap.
This system enables you to fully study the functional complexity of biological systems at the single-cell level.

For inquiries on collaborative research contact Akifumi Kiyota a-kiyota [at] kumamoto-u.ac.jp

International Core-facility of Advanced Life Science