September 10 2018
The "D5 Medical & Life Science Seminar" course will be offered by International Research Center for Medical Sciences (IRCMS). It will run from May 2018 to March 2019, with lectures given by scientists affiliated or collaborated with the IRCMS. The course theme for this academic year is "Basic research for understanding disease mechanisms". The lectures will be given once a month, in English, and by leading scientists in the relevant research field. Students will be taught: 1) how normal physiological functions are maintained in human hematopoietic, vascular, immune, reproductive and nervous tissues and organs; 2) how abnormalities in these systems (e.g., cancer) are studied using experimental models; 3) cutting-edge technologies (including single cell level imaging and omics analysis) used for mechanistic understanding of these abnormalities; 4) efforts and progresses in finding cure for human diseases associated with these abnormalities; and 5) importance of understanding disease mechanisms using
Date : October 31, 2018 (Wed)
Time : 17:30 -
Venue : IRCMS 1F Meeting Lounge
Speaker : Pilhan Kim Ph.D.
Title : Real-time Intravital Microscopy : In Vivo Live Cell Imaging
Over the recent years, intravital laser-scanning microscopy has demonstrated dynamic 3D visualization of various biological processes in a living subject, in vivo. It has been utilized to monitor gene expression, protein activity, drug delivery, cell trafficking, cell interaction, and physiological response to external stimuli in a live animal in vivo, which has provided unprecedented insights those were impossible to obtain with conventional microscopy techniques for static 2D conventional ex vivo and in vitro observation.
In this talk, a custom-built real-time laser-scanning intravital confocal/two-photon microscopy system will be introduced. The imaging system has been extensively optimized for in vivo cellular-level imaging of internal organs in a live animal model. Intravital microscopic imaging of various organs including skin, liver, spleen, pancreas, kidney, small intestine, colon, retina, lung, heart, lymph node, and bone marrow will be briefly introduced. Subsequently, recent studies utilizing the intravital imaging technique to investigate dynamic cellular processes such as transmigration of T and B lymphocyte through the perivascular channel in lymph node high endothelial venules will be introduced.