【Publications】Mobilization dynamics of bone marrow hematopoietic stem cells during hematopoietic regeneration

July 23 2024

Labs: Terumasa Umemoto

Paper information

Title:

Mobilization dynamics of bone marrow hematopoietic stem cells during hematopoietic regeneration

Johansson A, Khalilnezhad A, Takizawa H, Mizuno H, Suda T, Umemoto T.

Exp Hematol. 2024 Jul 13  doi: 10.1016/j.exphem.2024.104281.

URL: https://www.exphem.org/article/S0301-472X(24)00140-1/abstract

Highlights

• Endogenous BM HSCs are mobilized to spleen during hematopoietic regeneration.
• Early-phase HSCs passively egress from BM through attenuation of their retention.
• Late-phase HSCs gain greater cell-autonomous motility independent of chemotaxis.

Abstract:

Under stress hematopoiesis, previous studies have suggested the migration of hematopoietic stem cells (HSCs) from bone marrow (BM) to extramedullary sites such as spleen. However, there is little direct evidence of HSC migration from BM to spleen. Here, we induced myeloablation via 5-fluorouracil (5-FU) and showed the direct evidence of HSC migration from BM to spleen during hematopoietic regeneration via a photoconvertible fluorophore. Moreover, during steady-state, HSCs preferentially migrated to BM rather than spleen, but during hematopoietic regeneration HSCs preferred to spleen as a migration site equivalently or greater. Furthermore, in the early phase, HSCs egressed from BM through the attenuated HSC retention. However, HSCs in the late phase gained significantly enhanced cell-autonomous motility, which was independent of chemotaxis. Collectively, HSC mobilization from BM prior to the migration to spleen was dynamically changed from passive to active events during hematopoietic regeneration.