【Publications】In vivo two-photon calcium imaging of cortical neurons in neonatal mice

May 9 2023

Lab: Hidenobu Mizuno

Paper information

Tile:

In vivo two-photon calcium imaging of cortical neurons in neonatal mice

Takamitsu Egashira, Nao Nakagawa-Tamagawa, Elvira Abzhanova, Yuzuki Kawae, Ryoko Koitabashi, Hiromi Mizuno, Hidenobu Mizuno

STAR Protocols  2023 Apr 27;4(2) doi: 10.1016/j.xpro.2023.102245

Highlights

・Preparation of mice by labeling sparse cortical neurons using GCaMP

・Cranial window surgery procedure for in vivo 2-photon imaging of neonatal brain

・Single-cell level in vivo calcium imaging in neonatal brain

・Quantitative analysis of activity correlation

Abstract

In vivo calcium imaging is essential to elucidate unique synchronous activities observed in the developing brain. Here, we present a protocol to image and analyze activity patterns in neonatal mouse neocortex in a single-cell level. We describe steps for in utero electroporation, cranial window surgery, two-photon imaging, and activity correlation analysis. This protocol facilitates the understanding of neuronal activities and activity-dependent circuit formation during development. For complete details on the use and execution of this protocol, please refer to Mizuno et al. (2014),¹ Mizuno et al. (2018a),² and Mizuno et al. (2018b).³.

Graphical abstract