Protocol to Establish Cancer-Associated Fibroblasts from Surgically Resected Tissues and Generate Senescent Fibroblasts

June 22 2021

Lab: Takatsugu Ishimoto

Paper information

Title:
Protocol to Establish Cancer-Associated Fibroblasts from Surgically Resected Tissues and Generate Senescent Fibroblasts

Yasuda T, Koiwa M, Yonemura A, Akiyama T, Baba H, Ishimoto T*. (*corresponding author)

STAR Protocols 2021 May 26;2(2):100553. 

doi: 10.1016/j.xpro.2021.100553.

Highlights

• Detailed protocol for extracting primary fibroblasts from human gastric cancer
• Achieve the high isolation rate of fibroblasts by the current method
• Method to induce senescent fibroblasts by inflammatory cytokines

Summary

Accumulating evidence suggests that the malignant behavior of cancer is influenced by stromal activity in the tumor microenvironment. Cancer-associated fibroblasts (CAFs), which are the main component of the cancerous stroma, play an important role in cancer development. Here, we describe a protocol to establish CAFs from surgically resected tissues. CAFs could be a vital tool for understanding the microenvironment and its impact on tumor progression and metastasis. Moreover, we generated inflammation-induced senescent fibroblasts that more closely mimic the tumor microenvironment.

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