June 22 2021
Lab: Takatsugu Ishimoto
Protocol to Establish Cancer-Associated Fibroblasts from Surgically Resected Tissues and Generate Senescent Fibroblasts
Yasuda T, Koiwa M, Yonemura A, Akiyama T, Baba H, Ishimoto T*. (*corresponding author)
STAR Protocols 2021 May 26;2(2):100553.
Accumulating evidence suggests that the malignant behavior of cancer is influenced by stromal activity in the tumor microenvironment. Cancer-associated fibroblasts (CAFs), which are the main component of the cancerous stroma, play an important role in cancer development. Here, we describe a protocol to establish CAFs from surgically resected tissues. CAFs could be a vital tool for understanding the microenvironment and its impact on tumor progression and metastasis. Moreover, we generated inflammation-induced senescent fibroblasts that more closely mimic the tumor microenvironment.
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