March 24 2023
We would like to inform you that the 91st IRCMS seminar has been scheduled as below.
* This IRCMS seminar is open to everyone.
Date : April 7, 2023 (Friday)
Venue : Onsite & Online
IRCMS Lounge & Zoom
Time : 16:00-17:00
Speaker : Dr. Ruby Huang
National Taiwan University
Title : Spatial Profiling of Intra-tumoral Heterogeneity in Ovarian Clear Cell Carcinoma
Complex architecture of (sub-)clonal heterogeneity in the tumors arises from both the intrinsic factors from the tumor cells and extrinsic factors from the tumor microenvironment (TME). There exists context dependent composition of the clonal heterogeneity among different tumor types. Here, we address this heterogeneity in ovarian clear cell carcinoma (OCCC), a gynaecologic cancer showing high incidence in East Asia with critical unmet needs for novel therapeutic strategies. This talk will cover how the heterogeneity of OCCC has been deciphered along with the technology evolution of omics profiling from bulk to spatial analysis. OCCC showed distinct epithelial and mesenchymal subtypes and distinct immune-related gene expression signatures (irGES) at the bulk transcriptomic level. Immune-hot tumor features in early-stage OCCC were identified to be associated with a CTLA-4 subtype, an increase of spatially resolved increased tumor infiltrating immune cells (TIIs), and a papillary tumor morphology. The metastatic trajectory associated with clonal heterogeneity was explored in advanced-stage OCCC by the spatial profiling of OCCC tumors from the primary ovarian and multiple metastatic sites at both the protein and the transcriptomic levels. Certain metastatic sites of OCCC exhibits higher degrees of ITH than the primary site. An EMT gradient was found in the colonic metastasis of an advanced OCCC patient at both the protein and the transcriptomic level leading to the stromal interface of the tumor invasive front. The change of oxidative phosphorylation in OCCC might be associated with the clonal heterogeneity in OCCC.
Flyer: (Click to enlarge)