June 16 2022
The "Joint Invited Speaker Seminar Series for Future Medicine" will be co-hosted by Korea Advanced Institute of Science and Technology (KAIST) GSMSE and International Research Center for Medical Sciences (IRCMS). It will run from May 2022 to September 2022, with lecture given by scientists who are affiliated with IRCMS or in collaboration with reseachers at IRCMS. The lectures will be given three times a month, in English, and by leading scientists in the relevant research field.
Date : July 6th (Wed)
Time : 10:00 (JST)
Speaker : Dr. Ji Min Lee
Graduate School of Medical Science and Engineering,
Korea Advanced Institute of Science and Technology
Title : Controlling nuclear translocation of drug machinery by epigenetic engineering
MicroRNA-dependent mRNA decay plays an important role in gene silencing by facilitating posttranscriptional and translational repression. Inspired by this intrinsic nature of microRNA-mediated mRNA cleavage, here, we describe a microRNA-targeting mRNA as a switch platform called mRNA bridge mimetics to regulate the translocation of proteins. We applied the mRNA bridge mimetics platform to Cas9 protein to confer it the ability to translocate into the nucleus via cleavage of the nuclear export signal. This system performed programmed gene editing in vitro and in vivo. Combinatorial treatment with cisplatin and miR-21-EZH2 axis-targeting CRISPR Self Check-In improved sensitivity to chemotherapeutic drugs in vivo. Using the endogenous microRNA-mediated mRNA decay mechanism, our platform is able to remodel a cell's natural biology to allow the entry of precise drugs into the nucleus, devoid of non-specific translocation. The mRNA bridge mimetics strategy is promising for applications in which the reaction must be controlled via intracellular stimuli and modulates Cas9 proteins to ensure safe genome modification in diseased conditions.
This seminar will be held via Zoom.
Please register at the link below if you would like to participate.https://docs.google.com/forms/d/e/1FAIpQLSenZyyWw2h4XA4GhJUwaoMbZb95CNstqAFWcWkHiN451rDwMg/viewform?usp=sf_link