Name: Ananya Babu   
            Central University of Punjab, Bathinda

Visiting Period: January 14^th (Wed), 2026 - February 28^th (Sat), 2026

Country: India

Lab: Takahashi Lab

Q1. Summary of the internship (Description of the research conducted, aim, introduction of lab, and things you learned)

I completed my internship at the International Research Center for Medical Sciences (IRCMS), Kumamoto University, from January 14, 2026, to February 28, 2026. Under the expert supervision of Professor Yuta Takahashi, I engaged in research centered on epigenetic inheritance. Epigenetic inheritance refers to the transmission of information from one cell generation to the next or from parent to offspring that does not involve changes to the underlying DNA nucleotide sequence. Instead, this inheritance is mediated by molecular "tags," such as DNA methylation, which act as a regulatory layer that dictates whether genes are turned "on" or "off." By studying these processes, we can understand how cells maintain their identity and how environmental factors can lead to stable, long-term changes in gene activity without altering the genetic code itself. The primary objective of my internship was to generate locus-specific, endogenous protein-degradable knock-ins for the DNA methyltransferases DNMT1, DNMT3a, and DNMT3b using the CRISPR-Cas9 system. By integrating a BSD-P2A-dTAG selection cassette at these specific genomic loci, the study aims to establish a robust cellular model that allows for the rapid and reversible depletion of these enzymes. This approach provides a "switch-off" mechanism, enabling the precise investigation of their individual and collective roles in DNA methylation and epigenetic regulation by allowing us to study the immediate cellular consequences of their loss in real-time.

Q2. What did this experience do for you with respect to your specific career development directions?

Beyond technical skills, this internship experience has significantly shaped my career trajectory by working on this project, I have gained a specialized understanding of how to manipulate epigenetic regulators to study cellular mechanisms. This internship has not only solidified my identity as a molecular biologist but has also provided the international research exposure necessary to excel in the global scientific community.

 

Being part of a world-class research environment at Kumamoto University allowed me to observe the high standards of discipline and collaborative spirit that define Japanese scientific research. Living and working in Kumamoto City served as a bridge between my previous academic background and the global research community, helping me develop the adaptability required to thrive in diverse, international work environments. Beyond my time in the laboratory, living in Kumamoto was a wonderful adventure. I really enjoyed exploring the local culture by visiting places like Kumamoto Castle and the zoo. I also spent time walking through the Shimatori shopping arcade and trying many different types of local food. These experiences helped me feel comfortable in a new environment and taught me how to adapt to life in a different country, which I believe will be very helpful as I continue my career in international research settings.

Photo2: Explored the beautiful kumamoto streets

Message to prospective students

I would highly recommend the IRCMS Internship Program at Kumamoto University to any student looking to bridge the gap between academic theory and professional research. The program provides a unique opportunity to contribute to significant projects. My experience was greatly enhanced by the mentorship of my supervisor, Professor Yuta Takahashi. His guidance was instrumental in helping me navigate the complexities of the research project and provided me with invaluable insights into professional scientific rigor. Working at the IRCMS offers an immersive, international research experience that is essential for your development as a global researcher.

Photo3: with Takahashi lab members