April 23 2019
We would like to inform you that the 48th IRCMS Seminar has been scheduled as below. We would be pleased to see many of you participating in the seminar.
Date : May 24, 2019 (Fri)
Time : 16:00-17:00
Venue : IRCMS 1F Meeting Lounge
Speaker : Naihe Jing, Ph.D
Professor and Principal Investigator
Shanghai Institute of Biochemistry and Cell Biology
Chinese Academy of Sciences
During postimplantation development of the mouse embryo, descendants of the inner cell mass in the early epiblast transit from the naïve pluripotent state to the primed pluripotent state. Concurrently, germ layers are formed and cell lineages are specified, leading to the establishment of the blueprint for embryogenesis. Fate mapping and lineage analysis studies have revealed that cells in different regions of the germ layers acquire location-specific cell fates during gastrulation. The regionalization of cell fates heralding the formation of the basic body plan is conserved in vertebrate embryos. Knowledge of the molecular regulation that underpins the lineage specification and tissue patterning is instrumental for understanding embryonic programming and stem cell-based translational study. However, a genome-wide molecular annotation of lineage segregation and tissue architecture of the post-implantation embryo has yet to be undertaken. Here, we reported a spatially resolved transcriptome of cell populations at defined positions in the germ layers during development from pre- to late gastrulation development. This spatio-temporal transcriptome provides high resolution digitized in situ gene expression profiles, infers the molecular genealogy of tissue lineages and defines the continuum of pluripotency states in time and space. The transcriptome further identifies the networks of molecular determinants that drive lineage specification and tissue patterning, highlights the role of Hippo/Yap signaling in germ layer development, and uncovers the significant contribution of visceral endoderm to the endoderm in the early mouse embryo.